Studies on Coxsackie B₅ Virus by Tissue Cultures

Propagation of Group a Coxsackie Viruses in Tissue Cultures

In contrast with the 6 Group B Coxsackie viruses which readily induce a cytopathic effect (CPE) in tissue cultures, many of the Group A viruses have either failed to alter visibly cells propagated in cultural systems, or have required "adaptation" for such an effect. In the course of studies made during the last two years we have obtained CPE on primate cells cultured in vitro for all except 7 ...

Studies on Persistent Infections of Tissue Cultures

Inoculation of the MCN and Lung-To lines of human cells in continuous culture with Newcastle disease (NDV), mumps, or 6-6 viruses led to slight cytopathic effects (CPE) if the multiplicity of infection exceeded one. On second passage or with smaller initial inocula no CPE became apparent. The viruses multiplied, however, as determined by titrations in HeLa cultures or chick embryos. Indeed, per...

Comparative Biochemical Studies on Normal and on Poliomyelitis Virus-infected Tissue Cultures

Experimental evidence is presented for drastic changes in phosphomonoesterase activities of tissue cultures, brought about by infection with poliomyelitis viruses. Acid phosphatase activity went through a maximum before decreasing almost to zero level. Alkaline phosphatase activity diminished progressively to zero, then with disruption of the cells attamed normal levels. Various aspects of the ...

Myocarditis and Croup Caused by Coxsackie Virus

Myocarditis has long been recognized as a complication of rheumatic fever, diphtheria, scarlet fever and whooping cough. It also occurs in association with poliomyelitis and more rarely with other virus infections such as influenza, mumps, psittacosis, infectious mononucleosis, primary atypical pneumonia, varicella, measles, infectious hepatitis and yellow fever (Lyon, 1956; Silber, 1958). Myoc...

Studies on the replication of African Horse Sickness Virus in two different cell line cultures